Reporter

Part:BBa_K823039:Design

Designed by: Tamara Cikovic   Group: iGEM12_LMU-Munich   (2012-09-08)

gfpmut1 (B. subtilis optimized + Freiburg standard)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 197


Design Notes

We designed this part in the Freiburg standard for N- and C-terminal fusions.


Source

Plasmid [http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=nucleotide&dopt=GenBank&list_uids=306491148 pGFPamy]. In contrast to the reference below, the plasmid contains gfpmut1 (not gfpmut3).

References

[http://www.ncbi.nlm.nih.gov/pubmed/20600285 Bisicchia(2010)]